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Cell lysates were frozen immediately upon collection; following thawing, they were clarified by centrifugation at 10,000 g for 5 minutes at 4 °C prior to use.
Once patients agreed to be interviewed, the interviewer explained who they were, clarified the aims and objectives of the study and assured participants of anonymity and data confidentiality.
Proteins were obtained by incubating cells with the provided cell lysis buffer with protease inhibitors, and immediately they were clarified by centrifugation at 18,000 ×g at 4°C for 2 min.
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"We tried it because someone left the butter on too long when they were clarifying it," he said.
In 2009, in line with the Ecclesiae Unitatem act, Pope Benedict XVI tried to reconcile the SSPX with the Church, but stated that "the doctrinal questions obviously remain and, until they are clarified, the Society has no canonical status in the Church and its ministers cannot legitimately exercise any ministry".
Three out of five Americans are often sceptical of poll results (according to a poll, of course), and there have been numerous comments that the polls are confusing more than they are clarifying.
In so doing, they are clarifying an existing need while applying new technology.
Lysates were clarified by centrifugation.
All doubts were clarified.
They have been clarified by successive rule changes.
The arguments about that have not really changed, but they have been clarified by the Paris massacres and other recent atrocities in Europe, Africa and the Middle East.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com