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They further intercrossed in the center of the femoral head.
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c-myc fl / fl mice were mated with mice containing a single copy of the WAPiCre transgene and pups were further intercrossed.
An additional ten to fifteen F2 plants from each population were grown in a greenhouse for intercrossing, yielding ~2000 intercrossed F2 lines that have yet to be advanced further; intercrossing was intended to increase recombination and thus genetic power without the need for correspondingly larger populations.
For animal systems, insertional mutagenesis usually produces a heterozygous individual and then homozygous mutants by further intercrossing.
Further intercrossing generates an advanced intercross line (AIL), which increases the genetic resolution and allows for fine mapping of QTLs identified in a genome wide linkage analysis.
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They were intercrossed with wild-type C57BL/6 females and 4 showed germline transmission.
The WT and the ST2−/− mice were kept in two locations (Kragujevac, Serbia and Rijeka, Croatia) and they were intercrossed and typed.
Then they were intercrossed to obtain Cxcr4 flox/flox/ Lck-Cre, Cxcr4 +/+/ Lck-Cre, and Cxcr4 flox/flox mice on the DBA/1J background.
The founder μMT mouse was kindly provided by Dr Werner Müller (Institute of Genetics, Cologne, Germany), which we backcrossed to B10.Q for 13 generations before they were intercrossed for the experiment.
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