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padellus, mahalebellus, rorrellus, yanawaganus, and eurinellus, Thus, for our sequences these two restriction sites are not diagnostic between Y. malinellus and Y. cagnagellus + Y. padellus, but rather between Y. padellus and the other two taxa.
Thus, for a given DNA sample, the full methylation of the internal cytosines and hemimethylation of the external cytosines at the assayed CCGG sites can be unequivocally distinguished [20], [23] using these two restriction enzymes.
The SUMO-I sequence was obtained by PCR amplification and inserted between these two restriction sites, giving vectors pET-FPSG.
Thus, for a given DNA sample, the full methylation of the internal cytosine, or hemi-methylation of the external cytosine at the assayed CCGG sites, can be unequivocally distinguished [ 23, 25] using these two restriction enzymes.
According to the presence or absence of these two restriction sites, two alleles have been defined: the common "wild-type" allele (RasI+ /PstI−), known as c1, and the variant allele (RasI− /PstI+) known as c2.
These two restriction systems contained in a single M18 genome can be predicated to assist this strain in digesting foreign genetic material [ 36], which may explain why P. aeruginosa M18 has proven to be recalcitrant to the introduction of foreign DNA and to keep the strain isolated from others within rhizosphere niches.
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Network coding is a technique that exploits the broadcast of the wireless channel and can effectively address these two restrictions to improve network performance.
The relative merits of these two restrictions are weighed by Tennant (2002).
But given these two restrictions, the Principal Principle seems like a good constraint.
These two restrictions are expressed by the inequalities (iii) and (iv).
In our work, we remove these two restrictions and find seedless matches (in addition to the seed matches) throughout the gene regions.
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