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As shown in Figure 3A (filled square), these transposition cells were detectable 6 hrs after the temperature shift and increased in R+ culture.
However, in the R− culture, we could not sensitively measure these transposition products because of the growth of the cells that had lost the plasmid (Figure 3A, open triangle in the upper panel and open square in the lower panel).
The number of these transposition clones and the transposition frequency (number of double resistant cell per ml at 42°C/number of viable cell per ml at 30°C) (Figure 3B, lower panel) were comparable between R+ and R− cultures at hour 12.
In contrast, dogs experienced major difficulties to follow an object's invisible trajectory in these transposition tasks.
These transposition events must have occurred either in the F2 and F3 generation or in somatic tissue of the F4 plants.
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These transpositions of Bach's are never mechanical".
These transpositions are lineage-dependent.
Based on the location of the insertions and the adjacent genes, we have subdivided these transpositions into four types (A-D) (Fig. 3, 4, 5, 6).
Many of these transpositions will result in loss-of-function mutations, which are powerful tools for biologists to analyze gene function.
Hence, Class II has been divided into two subclasses depending on the number of DNA strand cuts at the donor site, which reflects these different transposition mechanisms.
In these cases, transposition has been supported using parsimony arguments (one transposition explaining a change with fewer steps than three inversions) or using the existence of inverted- and direct-repeat sequence motifs near the boundaries of rearrangements [ 33].
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