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These subsets show very limited overlap [7].

These subsets show transcriptional and genetic differences and seem to be associated with the different age groups (infants vs. adults; [ 14]) that exist within the SHH subgroup and with the presence of P53 mutations [ 18].

Interestingly, the comparison of these subsets showed a continuum in the expression of some NK receptors, with a significant decrease of CD62L, CD27 and granzyme-K levels (Fig. 6A), with the progressive increase in markers associated with maturation, including ILT-2, CD57, Siglec-9 and FCRL6 (Fig. 6B).

These subsets showed similar expression of costimulatory molecule CD86, the lack of CD80 and an enhanced expression of HLA-DR detected mostly on CD14++CD16+ monocytes.

Functional analysis of Gene Ontologies of these subsets showed increased expression of male cellular and metabolic processes, whilst decreasing expression of phosphorylation-related DEGs (Additional File 6, Table S6, P < 0.01).

The pm networks from the subsets showed a similar level of shared edges (23×106, 72%) (Table 5).

These three phenotypically defined monocyte subsets show different functional properties, such as patterns of cytokine secretion and chemokine receptor expression, and migratory properties into normal and inflamed tissue.

Different monocyte subsets show distinct inflammatory cytokine profiles and differentiation potential under steady-state and inflammatory situations.

Both network subsets show substantial alignment with the conversation spikes in the total volume count.

Both CD16hi and CD16lo monocyte subsets show similar ΔT2 when incubated with CLIO-MCSFR.

The basal-like and ERBB2 subsets show the worst clinical outcome [ 3, 4].