Interestingly, some of these localisations are only observed in specific cell lines, indicating that the variants localising to these cellular sites are potentially tissue specific.
Some examples of these localisations are shown in Fig. 2.
These localisations are thus likely to identify new mitochondrial factors.
These localisations are important for its function(s).
Thus, these localisations identify novel nucleolar factors in Drosophila.
These localisations were faithfully reproduced in uninduced gs3T3 cells transfected with single expression constructs (data not shown) and in MIF-induced stable gs3T3 cells.
Because all proteins in Category III are F-actin binding proteins, this suggests that these localisations correspond to the time when actin is polymerizing on either sides of the midbody (Herszterg et al., 2013b; Morais-de-Sa and Sunkel, 2013b).
In addition to these localisations at stage 5 to 10, we found a few insertions whose expression is not detectable at stage 5, but are clearly extracellular at stage 11 and/or stage 16 (supplementary material Table S1).
As we show here for Irgm2 and as already shown for Irgm3 (Taylor et al, 1997) and Irgm1 (Martens et al, 2004), these localisations were unaffected by the inactivating GMS to GMN mutations in the G1 motif.
