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Thermic variation cycles were 94°C for 4 min, 30 cycles of 94°C for 1 min, 50 58°C (depending on the primer) for 1 min, 72°C for 1 min and an extension final step of 72°C for 7 min. PCR was performed on a MJ Research thermocycler PTC 100.
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Following UV irradiation, thermic variations or oxidative shock, TIAR accumulates in so-called stress granules (SGs) that contain 43S pre-initiation complexes corresponding to aggregates of capped and polyadenylated mRNAs associated with small 40S ribosomal subunits.
In addition to the translational regulation of specific mRNAs, TIAR is involved in a broader translational repression mechanism which takes place in cells having to overcome environmental stresses such as UV irradiation, thermic variations or oxidative shock [10].
Interindividual variations in metabolic rate and in the thermic effect of food are important determinants of caloric expenditure, and an increasingly recognized factor, nonexercise activity thermogenesis (39), which includes activities like "fidgeting," may have genetic determinants as well.
Bottle variation?
Well, variation.
The variation was striking.
We found huge variation.
Variation must be abundant.
This variation is huge.
Geographical variation is huge.
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