Sentence examples for thermal settings from inspiring English sources

PCR condition is the following thermal settings: 30 cycles of 10 s initial denaturation step at 98°C, followed by 5 s annealing step at 55°C, and 3 min of extension step at 72°C using PrimeSTAR HS DNA polymerase (TaKaRa, Japan).

PCR was performed on Stratagene Mx3005P instrument using the following thermal settings: one cycle of 20 seconds at 95°C, and 55 cycles of 5 seconds at 95°C and 20 seconds at 60°C.

PCR was performed in a Taqman 7700 (Applied Biosystems) using the following thermal settings: one cycle of 15 min at 95°C, and 40 cycles of 15 s at 94°C, 30 s at 60°C and 30 s at 72°C.

PCR was performed with 100 ng of total RNA converted to cDNA per port in Taqman Universal PCR Master Mix (2 × ) (Applied Biosystems) in a 7900HT Fast real-time PCR System Applied Biosystemss) with a TaqMan Low Density Array Upgrade using the following thermal settings: 2 min at 50°C, 10 min at 94.5°C and 40 cycles of 30 s at 97°C, and 1 min at 59.7°C.

Open image in new window Fig. 3 Illustration of thermal cell settings.

Real-time PCR was performed on CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) with the following thermal cycler settings: 40 cycles of 30 s at 95°C and 30 s at 63°C.

The real-time one step RT-PCR employed the following thermal cycler settings: 30 min at 48°C, and 10 min at 95°C, followed by 50 cycles of 15 sec at 95°C and 1 min at 60°C.

The thermal desorption settings were optimised starting from an initial temperature of 150°C with a ramp rate 80°C min 1 up to 350°C and a hold time of 2 min.

The thermal cycler settings were as follows: 10 min at 50°C (cDNA synthesis), 5 min at 95°C (iScript reverse transcriptase inactivation), followed by 40 cycles for PCR cycling and detection of 30 s at 55°C.

Amplification reactions were carried out using a CFX Connect real-time PCR system with the following thermal profile settings: an initial step of 2 min at 95°C to activate the FastStart Taq DNA polymerase and then 40 cycles at 95°C for 15 s and 55°C for 15 s.

Amplification reactions were carried out on an ABI PRISM 7900HT sequence detection system (Applied Biosystems, Life Technologies) with the following thermal profile settings: an initial step of 10 min at 95°c to activate the FastStart Taq DNA polymerase, then 50 cycles of 95 °C for 10 s and 60 °C for 30 s.