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A direct, ambient ionization method has been developed for the determination of creatinine in urine that combines derivatization and thermal desorption with extractive electrospray ionization and ion mobility-mass spectrometry.
The products were ionized by electrospray ionization mode for their mass data.
Eluted peptides were ionized by electrospray ionization on a Q-TOF hybrid mass spectrometer (Micromass, Manchester, UK).
Separated peptides were ionized through electrospray ionization using the electrospray ion source (Proxeon Biosystems).
After electrophorethical separation, analytes are ionized (mostly by electrospray ionization (ESI) followed by MS analysis.
MS-based strategies usually involve transferring the active species into the gas phase within the MS detector, as well as ionizing the neutral compounds by electrospray ionization or desorption electrospray ionization strategies12,13,14.
Electrospray ionization mass spectrometry ESIMS were recorded on a Thermo Fisher LTQ Fleet instrument spectrometer.
The eluate was directly sprayed into the electrospray ionization (ESI) source of the mass spectrometer.
Here, we unravel the mechanism for this C-H functionalization reaction using desorption electrospray ionization, online electrospray ionization, and tandem mass spectrometry.
The metabolites were analyzed using electrospray ionization mass spectrometry operated in positive ion mode, ESI+.
This method has many similarities with electrospray ionization.
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