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Therefore, more stringent regulations and strategies are being introduced in order to facilitate appropriate allocation of land and efficient use of land, in order to produce both food and fuels [6],[7].
Therefore more stringent filtering conditions were applied than for the original PCR: genes with differential expression in more than 30% of the tumors at a tumor-to-normal ratio of 1.2 were removed from the dataset.
Skip3 thresholds are therefore more stringent than whole coding thresholds (supplementary table S3, Supplementary Material online).
Our analyses are therefore more stringent in that they are confined to sets of mRNAs that are more abundant than what is typically considered.
However, vast majority of the families included into the present study contains two melanoma patients only; therefore, more stringent selection criteria or subcriteria should be used in order to reduce chances that the identified melanoma families may simply represent a cluster of sporadic cases.
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The small and compact genome of Arabidopsis may lead to a higher probability for transformation-related side-effects and therefore require more stringent controls.
It is worth noting that our miRNA filter depends on the folding of MCE cognates from multiple genomes and; therefore, is more stringent than the direct folding filter that we applied to confirmed miRNA, which relies on flanking sequence from one organism only.
The sum was done over every non overlapping High Scoring Pair (HSP) (see BLAST documentation); the calculated percentage is therefore much more stringent than a calculation done on the first HSP only and being the division of similarity by alignment length, which can often result in spurious high percentages when HSPs concern conserved domains only.
Some researchers noted the high actual FDR of novel peptides and therefore employed more stringent filtering strategies, e.g. post error probability (Brosch et al., 2011) or separate FDRs for annotated and novel peptides (Branca et al., 2014).
The use of a prophylactic regimen of dexamethasone, ranitidine, and chlorphenamine, previously described, was not sufficient to prevent hypersensitivity reactions in two patients at 120 mg m−2, therefore a more stringent prophylactic regimen (Kwon et al, 2002) might be necessary.
We used two different thresholds for differential expression depending on the conditions that were compared, because our analyses showed that lower cutoffs in comparisons of RNA-seq data from microdissected samples and non-microdissected samples resulted in relatively large numbers of false-positives, therefore a more stringent cutoff was used.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com