Maize leaf discs (wt or Mon810) were extracted in sodium chloride (cell lysate) and CHELEX, a resin with a high affinity for divalent cations, thereby reducing problematic tertiary DNA structures that are known to become exacerbated in the presence of both Mg2+ and Ca2+, and limiting DNA degradation after cell lysis by inhibiting DNase activity, as this enzyme requires Mg2+ [ 45- 49].
Conversely, secure cash grants could make hiring and firing nationals less socially and politically problematic, thereby bringing citizen employment closer to the flexibility that employers currently enjoy with migrant workers [23].
Finally, by studying attitude changes we can see which aspects have changed and which aspects are still considered to be problematic, thereby pointing to recommendations for policy.
The informants perceived focussing on patients' problems to be problematic, thereby emphasizing the problems, rather than the patients' strengths to overcome weaknesses.
Second, PA in preschoolers occurs in short spontaneous bouts throughout the day, thereby making calculating PA time problematic.
Whether boundaries count as causes, and thereby explain evolutionary outcomes, is more problematic.
But Wall Street charged investors the same high prices associated with better-quality loans, thereby increasing their own profits on the problematic securities, according to a law enforcement official and executives with Wall Street companies.
Consequently, χ tends to increase with N and thereby displays increasing power to detect these problematic models.
Self-fertilization is problematic because it increases homozygosity, thereby reducing the contribution of overdominance to fitness and exposing deleterious recessives to selection.
12 First, a convention would define the various sorts of wrongful medicines accurately and thereby avoid misunderstandings caused by today's problematic or vague terminology (e.g., where countries seized good quality generic medicines as "counterfeit").
