This forms the initial basis for vCell isolation where all other components in the vCell are not exposed to receiving such messages - thereby isolating the components from broadcast messages.
We then applied slow voltage ramps to inactivate transient sodium currents (I NaT ), thereby permitting isolation of I NaP.
This phase is very flexible, with the possibility of using different panels of probes for different organisms of interest, thereby optimizing isolation according to current best knowledge about the organism and avoiding selection biases [ 33].
The rationale of the yeast-based model for CFTR-ΔF508 was thus to recapitulate its defective folding and trafficking by introducing an equivalent mutation into one of its yeast homologs, thereby allowing isolation of modifier genes thanks to the tremendous power of yeast genetics.
TN-positive cells can be isolated and maintained in culture after human islet isolation, thereby providing the possibility to further clarify its role and function in vivo as well as in the course of islet transplantation.
The latter group thereby experienced the isolation treatment but was also given the chance to re-acquire host CHCs (silverfish isolation control; colony 8).
In time, Communist pressure drove the Chetniks into tactical alliances with the Axis, thereby precipitating their isolation and defeat.
