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These sequences were used in the database of National Centre for Biotechnological Information (NCBI) (BLAST, http://blast.ncbi.nlm.nih.gov/) via BLASTN option [20] for possible matches, and thereafter submitted to GenBank for accession number.
The 24 rats were thereafter submitted to a 12 hrs.
Proteins were thereafter submitted to an overnight electrotransfer on a polyvinydilene difluoride Immobilon®-P transfer membrane (Millipore) at 4 °C.
Proteins were thereafter submitted to an electrotransfer on a polyvinylidene difluoride Immobilon®-P transfer membrane (Millipore) during 1 h and 30 mins at 100 V at 4 °C.
As recommended, it was thereafter submitted to the Commission nationale de l'informatique et des libertés (CNIL; http://www.cnil.fr/english/the-cnil/operation/ c1556) which ensures the protection of personal data.fr/english/the-cnil/operation/ c1556
In group AB, the calvaria defect was filled with autogenous bone obtained from ground calvaria bone harvested with the trephine; the same was performed for group ABL, which was thereafter submitted to LLL application.
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Single-walled carbon nanotubes (SWCNT) synthesized using the HiPco® process and purified thereafter were submitted to two covalent functionalization processes: i) a mild oxidation in a concentrated HNO3 solution using microwave irradiation and ii) a radical functionalization to graft methoxyphenyl groups.
In order to verify whether a maternal HF diet protects offspring from developing obesity and metabolic/endocrine alterations, adult offsprings born to HF or control dams and weaned on a chow diet were submitted thereafter to the obesogenic P diet.
Thereafter, plans were submitted to the regional or national health authorities/ministries for final approval.
For more than one-half (203, 54%) of the samples, the on-duty laboratory technician was required: 86 (23%) samples arrived within 1 hour before closing time, and an additional 117 (31%) were submitted thereafter.
Thereafter, it will be submitted as a public service announcement to various magazines that have donated space to PETA before.
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CEO of Professional Science Editing for Scientists @ prosciediting.com