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Thereafter, steps of random directions and specified amplitudes were presented, with intervals chosen from a clipped exponential distribution (1000 ms minimum, average 2000 ms).
We propose the following replication cycle (Figure 8), composed of an early phase between 0 3 h p.i. (steps 1 4) and of a late phase thereafter (steps 5 8).
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Concentrations were increased stepwise by 3% CO2 up to a concentration of 30% v/v, thereafter concentration steps of 5% CO2 were used.
Thereafter escalation steps were ⩽50%.
HIV viral load was determined at weeks 0, 8 and 16 of Step 1 and weeks 2, 4, 6, 8, 16 and every 8 weeks thereafter in Step 2 using the UltraSensitive Roche Amplicor HIV-1 Monitor™ assay in a central laboratory.
Thereafter the step to first patient application was made.
Thereafter, a step-wise decrease in PEEP allowed the detection of the minimum level that prevented the reappearance of atelectasis seen in the LUS images.
Thereafter, dehydration step was performed using 1 ml of accelerated ethanol concentration (15 min for each concentration) as follows: 50, 60, 70, 80, 90 and 100 (two times).
The second problem occurred shortly thereafter, around step 50, and was a result of the increase in efficiency as the simulation progressed.
Thereafter, each step was followed by washing 3 times with PBST for 3 minutes.
Thereafter, the step count increased only in the SA group with statistically significant difference between groups reached at day 6 (p = 0.02, Fig. 2).
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