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Total RNA was extracted from cells with TRIZOL reagent (InVitrogen, Cergy Pontoise, France) according to the manufacturer's recommendations and RNA were thereafter processed for RT-PCR, as previously described [45].

Next, uteri were dissected free, weighted and thereafter processed for histological examination.

The sections are incubated for 40 h at 4°C with the AT8 antibody (1 2,000) and thereafter processed for 2 h with the second biotinylated antibody (anti-mouse IgG).

Suggestions for new technologies and medicines were thereafter processed through three possible channels.

Thereafter, samples were processed for gelatin and in situ zymography.

Thereafter the cells were processed for RNA extraction.

Thereafter, hindlimbs were dissected and processed for histological analysis.

Thereafter, ticks were frozen at –80°C until processed for DNA extraction.

Thereafter, the ovaries were collected from rabbits and were processed for follicular population estimation and granulosa cells apoptosis.

Thereafter, the animals were sacrificed and the brains removed and processed for immunohistochemistry (Supplementary material and Fig. 1A).

Thereafter, the embryos were collected at different times (6, 24 and 72 h PR) and processed for laser capture microdissection.