Sentence examples for thereafter fixed from inspiring English sources

The left lung was weighed and thereafter fixed in 4% formalin and embedded in paraffin.

After a 24-h incubation, samples were washed in PBS 0.1 M, trypsinized and thereafter fixed with 1% w/v glutaraldehyde 4% w/v paraformaldehyde in PBS 0.1 M pH 7.2 for 2 h at 4°C.

First, the cells were stained with antibodies specific to stage-specific embryonic antigens SSEA-3 and SSEA-4 (monoclonal antibodies MC631 and MC813-70, respecthereafterhereafixedfixed, permeabilised and stained with Nanog-specific antibodies.

Cells were first stained for extracellular CD4-PE-Cy5 and CD25-PE (20 min at 4°C; BD Biosciences, San Jose, USA) and thereafter fixed and stained for intracellular Foxp3-APC according to manufacturers' recommendations (eBioscience, San Diego, USA).

Uterus was dissected free, weighted and thereafter fixed in 4% formaldehyde.

The tissue was thereafter fixed for 24 to 48 hours at 4°C in 4% paraformaldehyde, 1% glutaraldehyde, 0.1 M NaHPO4 pH 7.2.

Traditionally, scientists have treated the laws of physics as simply "given", elegant mathematical relationships that were somehow imprinted on the universe at its birth, and fixed thereafter.

PBMCs were immediately fixed thereafter using fixation/permeabilization buffer (True-Nuclear-kit, BioLegend) according to manufacturer's instructions.

For the MI and PI subgroups, the rates of SSI receipt increase slightly until the application period and basically remain fixed thereafter.

Yet, a different type of AChE1 insensitivity was described 10 years ago in a Culex pipiens population collected in Cyprus in 1987 and fixed thereafter as the ACE-R strain.

Three hours thereafter myofibers were fixed for subsequent immunostaining analysis to quantify Pax7+ cells.