Sentence examples for thereafter blocking from inspiring English sources

Thereafter blocking solution (1% BSA) was added and incubated for 2 hours at 37°C, followed by washing with PBST 3-4 times.

Transwell inserts and lower wells were blocked with 1% BSA/PBS overnight at 4 °C; thereafter, blocking medium was removed and lower wells were loaded with 300  μl of fMLF, fMMYALF or fMLKLIV in DMEM+0.5% BSA in concentrations ranging from 10−7 ℳ to 10−10 ℳ. U87 or U937-FPR1 cells were serum starved for 2 days.

In August, President Donald Trump proposed a ban on transgender individuals entering the military ― a proposal that was shortly thereafter blocked by a district court.

See eligibility criteria in table 1. Participants are stratified by centre and thereafter block randomised (random block size) to additional acupuncture or no additional acupuncture.

Thereafter, blocked membranes were incubated overnight at 4°C with monoclonal primary antibody for cTnI (sc-31655, Santa Cruz Biotechnology, 1 : 200), ssTnI (sc-8119, Santa Cruz Biotechnology, 1 : 200), α-MHC (sc-168676, Santa Cruz Biotechnology, 1 : 200), β-MHC (sc-168678, Santa Cruz Biotechnology, 1 : 200), and GAPDH (ab8245, Abcam, 1 : 7500).

A 100 μL/well of 1 in 1000 dilution of test plasma in 0.3% phosphate‐buffered saline (PBST) +EDTA was added after plates had been washed three times with 0.05% Tween in PBST, and thereafter blocked with 10% fetal calf serum (FCS /PBS (200 μL/well).

Coated plates were washed with PBS with 0.05% Tween-20 and thereafter blocked with SuperBlock in Tris-buffered saline for 5 minutes at room temperature followed by an incubation of mouse serum diluted 1 50 in TBS-0.05% Tween-20 for 2 hours at room temperature and overnight at 4°C.

Thereafter, blocks were mounted on plastic frames and cut using a microtome (Leica® RM 2155, Leica Instruments GmbH, Nussloch, Germany).

Thereafter, contralateral blocking was performed with the same method.

Russia thereafter successfully blocked American intervention against Assad on a number of occasions.

Bovine serum albumin (Sigma Chemical St Louis, MO, USA) was used for blocking, and thereafter different dilutions of control sera (purified mouse anti-CII antibodies), test sera, and positive and negative controls were added.