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The incorporation of predicted quantities in the input vector allowed for theoretical mutant N-linked glycan branching predictions without initial experimental analysis of protein structures.
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The improved potential of the E. coli wildtype is due to its membrane-bound transhydrogenase and its glycine cleavage system as shown by additional simulations with theoretical mutants.
Theoretical % mutant was calculated from TaqMan measured concentrations of mutant and wild-type template.
From a theoretical perspective, each mutant represents a possible fault that could be present in the original system (Copeland 2004).
Arising from the results of theoretical calculations, three mutants were prepared by site-directed mutagenesis and characterized by fluorescence analysis.
These analyses provide insights into the dynamics of proteins and allow for targeted design of functional mutants in theoretical and experimental studies.
When fermenting glucose as a sole carbon source, the RD mutant exhibited near theoretical ethanol yields (0.46 g g−1) during repeated-batch fermentations by recycling the cells.
Peptide ELISA results confirmed the improved binding affinity of novel scFv anti-p17 mutants from the theoretical calculations.
CI-inducing Wolbachia could also be displaced by sex-ratio distorting mutants, but empirical and theoretical evidence supports this idea only for induction of parthenogenesis and not for male-killing [26] [28].
One million bugs per gram of tissue left alive by the failing generic could increase its chances of selecting resistant mutants, making resistance a theoretical possibility [ 24].
Both theoretical simulation and experimental investigation indicated that the mutant strain had a much higher DBT biodegradation activity.
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