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For each candidate molecular formula explaining the root, we compute a FT, and then use the theoretical masses of all nodes in the FT as references to recalibrate the sample spectrum.
By comparing the masses of deglycosylated peptides in Figure 3A with their theoretical masses, m/z 1477.681, 2516.072 and 3530.644 were identified as deglycosylated peptides.
Table 2 lists also the theoretical masses and isoelectric points calculated for the proteins from their sequences, and the corresponding experimental values deduced from the 2-dimensionen electrophoresis gel.
This mass was double what was theoretically expected for bacterial TSPO (17 kDa) but ESI-MS/MS analysis (Fig. 5b) indicated a 92% recovery between experimental and theoretical masses and confirmed that this immunoreactive band was actually bacterial TSPO.
The signals were then compared with theoretical masses of tryptic peptides as predicted from a protein database of M. aeruginosa PCC 7806 (EMBL accession numbers AM778843 to AM778958) and the SWALL database using the taxonomy filter Eubacteria.
The assignments were based on molecular weights that were calculated from the mass spectra that matched the predicted theoretical masses of different H2A subtypes (primarily human) from known amino acid sequences.
Similar(11)
In the Standard Model, if all of those contributions were taken seriously and added up, the theoretical mass of the Higgs would explode beyond any reasonable value.
For decades, he explains, astronomers have been arguing about the theoretical mass limit of stars.
We compare the theoretical mass of each FT node with the observed mass of the peak.
Figure 11 The process of calculation of the final theoretical mass of the substances.
Let (mu (f)) denote the theoretical mass of the molecular formula f (either fragment or loss).
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