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The theoretical brightness of a fluorophore is defined as the product of the extinction coefficient and the quantum yield.
Moreover, the theoretical brightness of the fluorophore is not the only factor to consider when defining the detection sensitivity.
In this Review, we calculate the theoretical brightness of sensors by multiplying the quantum yield and extinction coefficient reported in the literature.
Fluorescent labeling reagents are an essential component of a huge industry built on sensitive fluorescence detection and reagents with close to maximum theoretical brightness are available in many different colors.
Two advantages of this system over the CFP-YFP platform are an increase in theoretical brightness and ability to excite the donor with a common 488 nm laser line.
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Despite decades of theoretical development of brightness models, it has been challenging to empirically evaluate assumptions underlying the models.
Aplanatic spherical/cylindrical CDOEs satisfying the sine condition are also capable of concentrating quasi-monochromatic diffuse light onto a flat target at the theoretical limit imposed by brightness conservation.
Based on its age and brightness, current theoretical models would have predicted it to be shrimpier and a brown dwarf.
The theoretical analysis of invariance properties of a logarithmic brightness scale under multiplicative transformations of the illumination field as well as multiplicative exposure control mechanisms is in excellent agreement with these psychophysical findings.
The best astrophysicists can currently do is use theoretical models based on observable properties like color and brightness.
The imaging system of the -PROGRA2 instrument allows to obtain maps of polarization and brightness of levitating dust clouds with a theoretical resolution of 10 μm per pixel.
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