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While all four men signed written confessions to espionage as a condition for their release — and then were immediately pardoned — some of the cases show how the definition of spying has grown murkier since relations have warmed between the United States and Russia.
Sections were washed twice with LCM staining solution to remove excess antibody and then were immediately dehydrated according to Histogene LCM Immunofluorescence Staining Kit protocol (Arcturus).
Supernatants were harvested after overnight and 6-day incubation and then were immediately tested for IFN-γ by using a commercial ELISA, according to the manufacturer's instructions (QuantiFERON-CMI, Cellestis Ltd, Carnegie, Victoria, Australia).
Control B and CLL cells were purified from peripheral blood mononuclear cells (PBMCs) by positive selection with CD19+ selection beads as suggested by the manufacturer (StemCell Technologies Inc ,Vancouver, Canada), and then were immediately used for RNA isolation or activation treatment.
The contents then were immediately discussed between doctor and patient.
The cells were incubated for 15 min at 37 °C and then were immediately washed in PBS and lysed in RIPA buffer containing protease and phosphatase inhibitors (Roche, Mannheim, Germany).
Similar(52)
Iheanacho of Nigeria hooks a shot wide and then is immediately subbed for Ighalo.
"Does anyone else find it faintly depressing that David Villa will be joining New York City, and then be immediately loaned out to Abu Dhabi's other soft diplomacy bauble, Melbourne City?
That's partly because tourists fly into the airport and then are immediately taken by speedboat to tourist islands that are just resorts, with no local inhabitants.
They can then be immediately frozen off with a metal probe cooled by a tank of carbon dioxide, available from any Coca-Cola bottling plant.
and then be immediately connected to a knowledgable expert.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com