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For instance, if the deleted gene encodes for a protein that is the only activator of another gene, then the expression level of the latter would be dramatically decreased in the deletion strain of the former as compared to the wild-type strain in which the regulator gene is intact.
Then, the expression level of PPAR γ was examined.
Then the expression level of Drp1 was analyzed by Western blotting.
Then the expression level of GAL4 regulated genes is measured to determine whether P1 and P2 interact.
If one of the genes becomes dysfunctional, i.e. it is knocked-out, then the expression level of that substance should be expected to be nill.
In addition, CNE1 were transiently transfected with different amount of pcDNA3.0-LMP1 or pcDNA3.0 (compensation to achieve equal amount DNA), then the expression level of LMP1 and phosphorylation of histone H3 at Ser10 were examined by western blot analysis.
Similar(51)
One hundred forty two microRNAs were randomly selected from 309 common microRNAs to the microRNA platforms, then the expression levels of these 142 microRNA in the human liver and prostate were measured by Taqman system.
Then, the expression levels of randomly selected representative miRNA species were further determined through stem-loop qRT-PCR.
And then, the expression levels of cytokines related with effector function of CD8+ T cells were determined by quantitative RT-PCR.
Then the expression levels of four candidate genes (KIAA0125, α-Catenin, β-Catenin, and Vimentin) in each cDNA sample were analyzed using primers listed below.
If the target genes are associated with the oncology of interest, then the expression levels of these genes will be a strongly biased estimate of their levels in the liver.
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