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He travels several times a year to California to designate specific blocks of vines in each vineyard that will be dedicated exclusively to City Winery; then refrigerated trucks are sent into the fields to transport the grapes.
The extracts were then filtered thrice through Whatman No. 1 filter paper to remove particulate matter and to get clear solutions which were then refrigerated (4°C) in 250 mL Erlenmeyer flasks for further experiments.
Then refrigerated at 4°C before proceeding to DNA extraction.
Samples were then refrigerated until used in the experiment, and for no longer than 36 h.
Poloxamer F127 was incorporated into MHB at a concentration of up to 30% which was then refrigerated overnight (4°C).
He spooned some chocolate into the mold then refrigerated it briefly.
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As many as 500 deaths were weather-related in the Chicago area then, and refrigerated trucks were used to handle the overflow from the morgue.
The serum was transported back to our field laboratory in a cooler and then kept refrigerated for up to two weeks before testing.
After 7 days in culture, cells were fixed in 4% Paraformaldehyde with 20 min incubation and then kept refrigerated in PBS till use.
They were then held refrigerated until shipment to CDC (Atlanta, GA).
Rice straw harvested in 2011 was chopped into small pieces of ~2 cm in length with a cutter, ground with a wet-disc mill (particle size: 0.7 ± 0.2 cm) and then kept refrigerated until use.
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CEO of Professional Science Editing for Scientists @ prosciediting.com