Sentence examples for then pretreated with from inspiring English sources

Jatropha curcas shells were extracted with water in a pilot-scale reactor and then pretreated with dilute sulphuric acid.

To confirm the roles of PI3K/Akt pathway, mice were then pretreated with wortmannin (0.7 or 1.4 mg/kg bw), a specific PI3K/Akt pathway inhibitor, before exposure to ethanol.

The substrates are then pretreated with argon plasma (flow rate is 5 sccm) at applied RF power of 40 W for 10 min in order to remove the native oxide present on the surfaces for enhancing film adhesion.

The cells were then pretreated with Compound A for 1 hour and then stimulated with doxorubicin for 24 hours, and DMSO treated cells served as controls.

The tissue arrays were then pretreated with proteinase K (DakoCytomation, Glostrup, Denmark) and washed with phosphate-buffered saline tween-20 (PBST).

For all experiments, rCMECs were grown to 80% 90% confluence and then pretreated with designated agents for 60 min prior to H2O2 exposure in fresh medium.

We then pretreated VSMCs with the Gi protein inhibitor pertussis toxin (PTX) and the Gq inhibitor YM254890, added Apelin-13 and looked for changes in Egr-1 expression.

To further validate PTEN expression mediated bosutinib-rescued tumorigenic phenotype in thyroid cancer cells, we transfected wild-type PTEN in FTC236-PTEN null cells and parallelly knockdown PTEN in FTC133-PTEN wild-type cells, then pretreated them with or without bosutinib before assaying their migration rate.

The differentiated cells were then pretreated for 24 h with ORF prepared in serum-free medium at concentrations of 1, 10 and 100 μg/mL.

The Round5 subline was then pretreated for 48 hr with 5-aza-dC or zebularine prior to administration of the GI50 dose of cisplatin.

The cells were then pretreated for 3 h with colcemid, a mitotic shake-off was performed, and the cells were treated for 6 or 12 h with 1  μg/ml Dox under colcemid pressure.