Sentence examples for then passage from inspiring English sources

Even then, passage came only after one market-rattling floor defeat.

Still, after the meeting with the governor, Mr. Silver seemed to indicate he was close to a compromise and then passage of a measure on borrowing with Mr. Bruno.

Then, passage of a war declaration by Congress would clear up all the legal and constitutional questions surrounding the current "police action" in Iraq; and finally, the prospect of actually declaring war would force both Congress and our society as a whole to think hard, avoid politically expedient vagueness, and finally figure out precisely who the actual enemy is.

Free-floating spheres were collected from flasks and dissociated into single-cell suspensions by pipette trituration then passage through a 40-μm cell strainer (BD Biosciences, San Jose, CA).

We then passage these viruses to select for functional variants, and use Illumina deep sequencing to determine the frequency of each HA mutation before and after this selection for viral growth.

The cells were then passaged and the attached passaged cells were then again treated with TI or silibinin/TI for another 24 h.

During rapid proliferation phase, cells were grown to 90% confluence then passaged 1 3 every 2 3 days.

These were routinely grown to 90% confluence then passaged as for primary cells.

Arid1af/f CreERT2 ESCs were cultured on gelatin-coated dishes, treated with either ethanol or 1 µM tamoxifen then passaged 24 h post treatment.

To extract RNA from PSCs, they were grown on feeder cells and then passaged under a feeder-free condition to avoid any contamination of feeder cells.

After selection, cells were allowed to recover for 3 7 days and then passaged onto a new plate (2000 cells per 6-well).