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The RNA was then obtained from 1 µg of DNA by in vitro transcription using the MEGAscript kit (Ambion).
The material properties of these die materials were then obtained from tension and impact tests.
A combined random-effects estimate was then obtained from the city-specific effect estimates.
Pedestrian crossing behaviour was then obtained from careful review of the videotapes.
The numerical velocity is then obtained from the discrete weak gradient of the numerical pressure.
The shift rates and the temperature shift factors can be then obtained from the model parameters.
Their constitutive relations are then obtained from observed data to complete the WPE nexus model.
The AR coefficients are then obtained from this instrumental variable sequence.
Fungal cultures were then obtained from these carbonate rocks and characterized by sequencing Internal Transcribed Spacers (ITS).
Amino-allyl amplified RNA was then obtained from total RNA using the Amino-allyl MessageAmp aRNA kit (Ambion, Inc., Austin, TX, USA).
A new piecewise bilinear representation at the next time level is then obtained from a two-step procedure.
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