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Cota et al. (2013) further reported that the mode of operation of their chimera was exactly similar to that of the parental enzymes.
Their chimera experiments show that these observations are not due to negative selection.
Z hang et al. (2006) reported a technique that significantly reduced the frequency of chimeras that they observed in their shotgun sequencing of cloned DNA from whole-genome-amplified single prokaryotic cells (without de-branching, their chimera rate was reported as 19.3%; with de-branching, it was reduced to 6.25%).
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Others were not so easily robbed of their chimeras.
Accordingly, since production of bacteriocins from synthetic bacteriocin genes is difficult to predict, further efforts should be performed for a more efficient genetically engineered production and functional expression of other bacteriocin synthetic genes or their chimeras by heterologous producer yeasts.
According to their results, chimeras in MDA can be divided into four types as shown in Fig. 2B.
Passages of DEN viruses or their derived chimeras in Vero cells [21], [22] have been shown to generate mutations specific for host cell adaptation, virus attenuation, or other properties yet to be characterized.
My primary recommendation is that the authors take their best 1-6-1 chimera carrying the SCRD and their best 6-1-6 chimera which loses SCR activity and perform the more quantitative kinetic assay as shown in Figure 2A and B. These data should be included in either Figure 5 or Figure 6. 4) The molecular docking of PS to the modeled structure in Figure 8D and E is premature and should be removed.
Another possibility is that the mechanism by which chtB knockouts achieve their success in chimera involves earlier fruiting.
Their relationship to the chimera of "race" is complex and hard to chart, but appears to be real enough.
(One sufferer wakes next to his wife in a panic, convinced that their relationship is a chimera and, despite decades of marriage, they are strangers).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com