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After hybridization, the crop genomic segments will be established in the wild background or eliminated by selection either through selfing of the hybrids or through backcrossing to the predominant wild plants, or a combination of the two processes.
It was notable that the most useful APA variants in our study were Arc1 in the cultivated background and Arc1, Arc4, Arc5 and Arc7 in the wild background and this is consistent with results from previous studies [ 19].
To introgress Q40-YFP transgene into the wild backgrounds, Q40Bristol males were mated with DR1350, JU258(Madeira), or CB4856(Hawaii) hermaphrodites, then five to seven F1 fluorescent hermaphrodites were mated with wild males, and this two-step cycle was repeated until over 35 generations of backcrosses to the wild background were reached.
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We argue that a collection of such lineages will provide an ideal system in which to test the agronomic utility of the wild backgrounds.
We generated the strains expressing Mcs10-3xHA in the wild type background, in Mcs12-His6 bandground and Mcs29-His6 background.
Western blots indicate, however, that the protein is present at a similar level in the mutant HP1 background as in the wild type background.
As shown in Fig. 6C, SOD2 overexpression produces more ommatidia with extra interommatidial cells in the rbf1 mutant background than in the wild type background (24% in wild type and 46% in rbf1 mutant).
Recombinant Inbred Lines with the crop genomic background for LG5 and the wild parental background for LG7 (5C–7wereexpectedctod to have the highest fitness.
In a previous study, we identified two genomic regions under selection in the RILs, one where the crop genomic background was selectively beneficial and one where the wild genomic background was selectively beneficial (Hartman et al. 2012).
There is also use of Rag -/- mice as the background for HOIL1 mutation interspersed with use of the wild type background.
In the dev mutant background, P nfsA :: mCherry expression was similar to that in the wild type background from 0-18 hours, but subsequently failed to accumulate to wild type levels.
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