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Sequence databases from the whole sequenced genomes of 1489 bacterial strains were downloaded from the NCBI.
Analysis always includes the whole sequenced fragments, thus both exons and intron in Pra Nsp-D2 and -D3.
Comparing total number of retrotransposons in rice genome (Table 2) and retrotransposons in Chromosome 10), we noticed that two families (Osr12 and 33) have more elements in Chromosome 10 than in the whole sequenced genome.
In sum, complex evolutionary history, especially with selective pressures that can cause parallel or reversal of specific loci, can cause difficulties with phylogenetic reconstruction even when the whole sequenced genome is available.
A medium-density genetic map (1422 markers) was used, which is much lower than what would be obtained with 60 K SNP arrays, and does not allow for linkage disequilibrium analyses, but it is dense enough for fine mapping of QTL using linkage analyses, and the selected markers cover the whole sequenced genome.
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But the whole sequence was pure performance.
"I've got the whole sequence in first editions, man.
The whole sequence had taken about thirty seconds.
The whole sequence is called an oxynitriding treatment.
The original script had left the scene vague, so Hymes had invented nearly the whole sequence.
"The whole sequence is sort of designed like an Ernst Lubitsch movie," he said to me.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com