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Exact(34)
We investigated the distribution and morphology of the neuronal structures in the human upper urinary tract using the whole mount preparation technique and confocal laser scanning microscopy.
The whole mount double in situ hybridization protocol and the probes are described [33].
The whole mount in situ hybridization protocol has been published previously [34].
Cardiospheres were fixed in ethanol/acetone and stained in the whole mount.
Volume renderings at the same threshold value depict the whole mount volume of liver vasculature (Panel D).
AR performed the whole mount in situ hybridization.
Similar(26)
(A and B) iWAT was processed for the whole-mount immunolabeling of STMN2.
Fiducial markers could not be introduced into the specimen after prostatectomy because they would be dislodged during sectioning of the whole-mount blocks.
iWAT was processed through the whole-mount immunolabeling of intended epitopes, and then subjected to the optical clearing procedure for imaging on the lightsheet microscope.
A similar approach can also be used to isolate large quantities of inner hair cells and selected supporting cells from the whole-mount cochlear preparation.
Two fixation conditions were used for the whole-mount immunohistochemistry: Bouin's [86] and methanol/acetone.
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