Sentence examples for the well solution from inspiring English sources

Crystals were harvested and protected in the well solution containing 30% (w/v) PEG3350 and cooled in dry nitrogen stream at 100 K for X-ray data collection.

Protein samples (12.5 mg/mL, 1 µL) stored in 25 mmol/L Tris-HCl, pH 8.0, 150 mmol/L NaCl and 5 mmol/L β-mercaptoethanol were mixed with well solution (1 µL) and equilibrated against the well solution (75 µL) in 96-well plates HR3-2711, Hampton Research).

The crystal was washed three times in the well solution and put in SDS-buffer.

For the hanging drop method, the protein drop is suspended above the well solution from a seal and makes no contact with the crystallisation plate.

The crystals were soaked in the well solution plus 10 mM NaSO4 for 10 minutes before flash-frozen in liquid nitrogen.

Crystallization experiments with varying concentrations of CaCl2, SrCl2 or BaCl2 in the well solution instead of MgCl2 did not yield satisfactory crystals.

In addition, for complexes with different metal ions the corresponding metal chloride salts were introduced into the well solutions at concentrations of 50 mM prior to crystallization drops being set up.

These were cryo-cooled in liquid nitrogen in the Morpheus well solution.

For cryopreservation, single crystals were soaked in the appropriate well solution supplemented with 26% (v/v) glycerol and then flash-frozen in liquid nitrogen.

The drop was equilibrated against well solution containing 30% PEG1000, 0.1 M succinic acid-phosphate-glycine (SPG) buffer pH 8.0.

Be sure to completely cover the well with solution.