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Microfluidic technology has been incorporated in sensor design to reduce the volume of samples and reagents.
The column lifetime, in terms of its ability to quantitatively derivatize FA to give highly fluorescent product, was investigated as a function of the volume of samples (20 ng/mL, FA) injected onto the CTH-packed reactor using phosphate buffer (0.04M, pH 3.4) as a carrier stream at a flow-rate of 0.25 mL/min and packed reactor temperature of 40°C.
The volume of samples in each well was brought up to 50 µl using the chloroplast lysis buffer.
The scale experiments were conducted with the volume of samples increased to 2 L using optimized parameters in the same buffer.
The limitations to carrying out exposure assessment included too few personnel, insufficient numbers of mercury monitors, and laboratories unable to handle the volume of samples.
Secondly, limited by the volume of samples, we did one-sample test and thus may underestimate the diagnostic value of all methods.
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The 24-hour mass concentration of PM2.5 is calculated as the difference in filter weight before and after sampling, divided by the volume of sampled air.
The volume of sample injected was 2 µL.
The volume of sample was brought up to 10 mL by the addition of methanol.
The volume of sample was 500 ml, and total time of experiment was 60 min.
The volume of sample was 100 ml, and all the temperature (25 ± 4°C).
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