Pellet volume was estimated and re-suspended into twice the volume of Loading Buffer.
77 Another potential option is to monitor the volume of loading to which a tendon is exposed (eg, number of jumps, hours of training, distance run, etc); however, there is an inherent inaccuracy in any such measurement utilised to date.
In summary, a serial connection of two large MAs can be used to increase the volume of loading material to up to 1,000 ml of cell culture SN.
I didn't set out to talk about climate volatility and weather disruption, but by the turn of the 21st century this warming seemed to be flavoring much of the weather I was tracking, turning up the volume of extremes, loading the dice for weather weirding.
Nonetheless, neither the volume of fluid loading nor the fluid balance was accurately recorded in the database.
The starting population theoretically represented 760,000 E2 and E6 amplicons, and 28,571 HMBS amplicons per 2 μl of NA6 template (the volume of template loaded in each PCR reaction), which equated to a load of 53 HPV16 copies per diploid genome.
The volume of medium loaded for protein separation was normalized to the numbers of cells on each plate at the time of media collection.
The supernatant was taken out and mixed with the same volume of loading buffer and then was analyzed with SDS-PAGE.
For Western blot analysis, the soluble and insoluble protein preparations were mixed separately in the same volume of loading buffer, separated on SDS/PAGE gels and then transferred onto nitrocellulose membrane.
The PCR products, 4 μL for each, were also mixed with the same volume of loading buffer (1 g/L xylene cyanole, 1 g/L bromophenol blue, in formamide), and resolved on an 8% polyacrylamide gel containing 8 mol/L urea using the Mini-VE system (Amersham Biosciences, San Francisco, CA, USA) at 120 V for 4 h.
Anti-T1, T2 and LD1, LD2 antisera were used to detect the four purified recombinant proteins and to probe native T1, T2 and LD1, LD2 proteins in crude S. japonicum extracts as follows: 1) 30 ng of recombinant T1, T2 and LD1 or LD2 protein was mixed in the same volume of loading buffer, separated on SDS/PAGE gels, and then transferred onto nitrocellulose membrane.
