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I was asked last week whether I thought the anticipated appearance of Michelle Obama on The View was calculated to soften her image and reach out to the stay-at-home moms who are fans of the show.
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The percentage of stained cells in the field of view was calculated as follows: five consecutive high-powered fields in each section were observed under a 400x optical microscope, and then the scores were averaged.
Afterward, the average number of vessels from the three fields of view was calculated.
Two-dimensional ARFI images were also acquired, and an average propagation velocity across the lateral field of view was calculated.
The number of cells per 10 fields of view was calculated from each structure, and data are presented as the mean ± 1 standard error of the mean.
The mean number of cells per field of view was calculated and averaged between duplicate transwells.
The average number of GFP cells per field of view was calculated for each coverslip.
The fraction of altered mitochondria, defined as number of mitochondria with disrupted cristae divided by total number of mitochondria in a view, was calculated.
Total hippocalcin-YFP fluorescence in a field of view was calculated for each frame during prolonged experiments and values of fluorescence in ROIs were normalized to the total fluorescence to compensate for protein photobleaching.
As a metric for the optical performance of this 1 1 telescope, the power mean with exponent 2 of the wavefront RMS at 9 points, uniformly distributed over the field of view is calculated.
Minimal important difference (MID) or the smallest score of change considered important from the patients' and clinicians' point of view were calculated using the distribution based approach.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com