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For the verification sample set, a unique blinding key was generated and provided exclusively to a third party reader (K.C ., unaffiliated with the study centers or SomaLogic, to score and report the final verification results.
Following institutional approval, each ICU in the verification sample provided a list of all blood cultures (BCs) performed over 3 months, and the case records of 5 20 patients with positive BCs.
For logistical purposes, if on arrival to their community a selected case was unable to participate in the verification exercise, a reported case (of the same lymphoedema severity and sex if appropriate) from the same or neighbouring community was included in the verification sample where possible.
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From both the training/test samples and the verification samples, there is a notably high correlation between the outcomes of ANN and IP and the actual results, which varies between 97.18% and 99.87%.
Thus to test if the effect is only specific to squamous cell carcinoma NSCLC (SqCC), six patient samples from adenocarcinoma NSCLC (AC) and small cell lung cancer (SCLC) patients respectively were included in the verification samples set as well as eleven SqCC patients.
As such, the first verification sample comparing paper listings to the electronic BCG Registry comprised 4,987 records.
With respect to data quality ascertainment in the second verification sample, 4,709 (94.7%) out of 4,972 vaccination certificates were found in the electronic BCG Registry.
Additional file 3: Estimated number of true cases and reported cases for the verification exercise sample size calculations.
We performed the preparation of standard solutions, quality control (QC) verification, sample preparation, and instrumental analyses as previously described (Calafat et al. 2004) with slight modification of the sample preparation as described herein.
A verification sample set consisting of 25% of all samples in the study was randomly selected and the identification of this set was blinded.
Table 1 presents, for both verification samples, the number of discrepancies and proportion of agreement per variable for "non-empty" fields among those verified.
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