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Ten One's diminutive accessory was small enough that it slipped easily into my carry-on laptop bag, and the various inserts for different types of devices were also easy to pack.
The target sequences were then cloned into the pFC62 episome using the XhoI and HindIII sites; successful cloning of the various inserts was confirmed by sequencing at the UC Davis Sequencing Facility.
For Gateway cloning, the various inserts to assemble were first cloned in the appropriate Multisite Gateway vectors.
For GoldenGate cloning, the various inserts to assemble were first PCR-amplified using Phusion polymerase (ThermoScientific) with primers adding BsaI sites on their extremities.
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Costs are also vastly different over the various insert types, with small GA being the cheapest, larger GA being more costly because of library inefficiencies, and fosmids being the most expensive according to direct library and sequencing costs.
To assess the value of the various insert size libraries for genome structure analysis, we determined the ability of each library to (1) physically cover the reference genome and overlap various repeat elements, (2) drive contig scaffolding, and (3) fix contig assembly issues in the current genome assembly (errors in contig order and orientation).
Moreover, the use of various inserts in the flow as conical nozzles, conical rings, V-nozzles and screw-tapes [18, 19] are usually found in the literature.
For microscopy, all strains containing the pMMB67 Gm vector control or with various inserts (biosensor or complementing clone) were grown overnight in the presence of 100 μM IPTG and 30 μg/ml gentamycin in LB medium.
The effect is lovingly achieved by means of various inserts (postcards, newspaper clippings, maps) and dense, handwritten marginalia.
Comparisons of the performance of plain membranes with membranes fitted with various inserts are reported.
Co-translational cleavage of the 2A sequence was validated to exceed 0.95 with various inserts.
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