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The values were normalized using the LOWESS algorithm.
The values were normalized using a LOESS method similar to the one implemented in the cellHTS2 R-package [ 14].
As before the enrichment scores were calculated using the hypergeometric distribution and the values were normalized using the background pool of transcriptome datasets.
To quantify the differences in the peaks of Ca2+ transients, the values were normalized using the formula (Fi− F0)/ F0 (where Fi is the value of fluorescence recorded during the stimulation and F0 is the basal value of fluorescence recorded).
The initial velocity was calculated from the slope of the linear range in the signal vs time plot for each assay, and then the values were normalized using the non-inhibitor control as a standard of 1 to obtain the relative initial velocity with standard deviation (SD) from at least three biological repeats.
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After scanning of the gene chips, images were analyzed and the expression values were normalized using the Affymetrix Microarray Analysis Suite (v5.0).
The expression values were normalized using cyclic loess normalization [ 25] with house-keeping probes up-weighted 100-fold.
In this and all subsequent experiments, the amplification efficiency of the PCR control was found to be uniform (data not shown) and the CT values were normalized using the ILC as shown in figure 2A.
The intensity values were normalized using the oligo package from R statistics software [ 48].
The CT values were normalized using the IQ5 Optical System Software version 2.0 (Bio-Rad, Veenendaal, The Netherlands).
The obtained values were normalized using the Gapdh1 gene as a reference.
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