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The used strain C. albicans ATCC 3153 was grown for 48 h at 37°C on Sabouraud Glucose agar containing Chloramphenicol (Fluka, Saint-Quentin Fallavier, France) to obtain a culture of synchronous stationary-phase yeast.
Therefore, this discrepancy most probably attributes to the difference of the used strain.
Local variations in treatment strategies exist depending on the choice of the veterinarian but due to the used strain collection this effect will be minimal.
M. brunneum has a worldwide distribution and is a natural pathogen of ants [ 55], yet the used strain was isolated from Austria and therefore lacks a local co-evolutionary history with all studied host ant populations.
These results show that the phenotypes generated in RNAi experiments can depend on the genotype of the used strain and we suggest that a proper documentation of the strain is an essential piece of information when publishing RNAi studies in any species.
Moreover, the development of colitis in mouse models has been shown to be affected by the genetic background of the used strain [i.e. some strains are intrinsically more susceptible to developing colitis (Büchler et al., 2012)] and the interactions with the gut microbiota [e.g. some models do not develop colitis in germ-free conditions (Hudcovic et al., 2001)].
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GW carried out isolation of the used strains, DNA extraction, PCRs, sequencing of meq and LTR genes and nucleotide alignments.
The less frequently used strain EMA is based on a response measurement using strain sensors.
The most commonly used strain of Bt (kurstaki, or Btk) targets only certain species of caterpillars.
Not used: Strain, Tomes.
C58 is one of the more widely used strains.
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