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The used cell types, cell viability, and possible construct applications are also reported.
We want to thank Gabriële Holtappels of the Department of Oto-Rhino-Laryngology oforhent University for performing the cytokine analysis and Karlien Van Wesemael for confirming the used cell line to be mycoplasma free.
One clear advantage of the cell sheet technology is the formation of native cell-to-cell and cell-to-matrix interactions which initiate the appropriate and inherent cell signalling cascades of the used cell types (Hashimoto et al. 2016; Mifune et al. 2013; Neo et al. 2016).
The used cell line collection was previously described in [9].
Computing half-maximal-inhibitory concentrations (Eq. (2), Materials and Methods) for PI-103 and BIBW-2992 (Figure 2C) shows no clear association between the genomic lesions and the single-agent activity of PI-103 with the used cell proliferation assay [19].
The used cell lines include U266, U266_SC, IM9, and RPMI8226 cell lines.
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