For those participants questioned about the use of loose larvae complete healing would have to have taken place over 17 weeks for them to choose larvae as their preferred option rather than hydrogel.
In summary, we describe the use of zebrafish larvae as a model to study epilepsy mechanisms.
Most of the characters used below rely on the use of mature larvae, 5th instar, or perhaps 4th.
FH performed growth rate assays and competition experiments and drafted the manuscript; LEB carried out the in vivo virulence bioassay and contributed to the manuscript; MV and AB developed the use of waxmoth larvae as a model host for P. aeruginosa, optimised the assays used and advised during manuscript preparation.
For all experiments that did not involve the use of tumour-bearing larvae, w larvae were used.
Thus, for our approach, the single-most limiting factor in screening potential is the time taken to manually sort and distribute Drosophila larvae into 96-well plate format, although the use of an automated larvae sorter has the potential to overcome this bottleneck.
Probable explanations include the use of different instars of larvae and assay times.
For registering gene expression patterns of 3-day-old larvae we generated a reference template (Randel et al., 2014) where we removed the bias present in the earlier reference, due to the use of a single larva as a starting template (Asadulina et al., 2012).
There is also increasing interest in the use of zebrafish embryos and larvae as a model organism for toxicity testing, ecotoxicology screening, and disease modelling, with a particular focus on drug screening assays (e.g. see Berghmans et al., 2008 and Redfern et al., 2008).
