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Spot numbers were automatically determined with the use of a computer-assisted video image analyzer with KS ELISPOT 4.0 software (Carl Zeiss Vision).
The use of a computer-assisted glucose regulation protocol Glucose Regulation for Intensive care Patients (GRIP was part of routine postoperative care.
Another important aspect of this study is the use of a computer-assisted methodology, which provided good repeatability and the capability to quantify reliably anatomical and kinematic features.
This randomized trial of 497 cardiac surgery patients showed that a single intraoperative high dose of dexamethasone leads to significantly increased lactate and glucose levels in the early postoperative period, with the use of a computer-assisted glucose control system.
Zahlmann et al [ 38] found a significant decrease in HbA1c values with a decision support system ('DIABETEX') and Horan et al [ 39] reported a significant improvement in the level of blood glucose with the use of a computer-assisted system designed to promote self-management of insulin.
Plates were incubated for an additional 2 h at 37 °C, and spot development was done as described in ref. Spot numbers were automatically determined with the use of a computer-assisted video image analyzer with KS ELISPOT 4.0 software (Carl Zeiss Vision).
Finally, images of the entire nerve cross-sections of the distal nerve (+0.5 mm to the distal coaptation side) were acquired by automatic multiple-image-alignment process with the use of a computer-assisted Olympus BX51 microscope and cellSense Dimension software (Olympus, Hamburg, Germany).
The remaining data were collected by independent research assistants blinded to the study allocation of the patient, using a computer assisted telephone interview method.
The use of audio computer assisted self interview in wave I and computer assisted self interview in waves III and IV of Add Health almost surely enhanced the candor of respondents when answering potentially sensitive questions.
The qualitative data analysis was undertaken using a computer assisted qualitative data analysis software: NVivo©.
The number of cells was estimated by optical fractionator probe using a computer assisted image analysis system with a Zeiss Axioplan 2 microscope connected to a digital camera.
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