Two PCR primers were designed to specifically amplify the unique sequences from serovar O3 K6 isolates.
The unique sequences in these tissue-specific splice variants may provide the potential targets for drug design and screening.
Three individual oligonucleotide probes (oligoprobes), complementary to the unique sequences of each toxin gene, were designed and immobilized on a surface of aldehyde-coated glass slides.
Within each sub fragment, the unique sequences had distinct HRM profiles (Figure 1A, 1B, and 1C).
Finally 22.8% (8990) of the unique sequences were found only as transcripts.
The unique sequences in CRISPR loci, "spacers", show a general divergence within a given species.
Sequences not identified in the genome were added to the genomic sequences to constitute the unique sequences set.
All identical sequences were compiled to generate a data set of the unique sequences with associated read counts.
The set that was found only in the genomic sequences included 40.8% (16,104) of the unique sequences.
One such distinguishing mark could be the unique sequences found in the centromeric or pericentromeric region of each homologue.
The resulting set of the unique sequences with associated 'read counts' is referred to as sequence tags.
