However, bacterial whole-genome sequencing (WGS) using NGS, by giving access to the whole genetic content of a strain, is the ultimate discriminatory sequence-based genotyping method and has already demonstrated its usefulness for epidemiological investigations, showing the rapid global transmission of infectious diseases [ 38, 58, 59] (Table 3).
However, the intron sequences are very different, so that the ultimate evidence, intron sequence similarity, is lacking.
To overcome these obstacles, we have coupled high-dimensional (Hi-D) FACS sorting with unique IgH multiplex PCR technologies, which allow inclusive amplification of IgH transcripts for each sorted B subset and ultimate sequencing of these sequences.
If specific sequences are the ultimate goal of genome sequencing, then more directed approaches would be more appropriate than our random GSS approach.
And that makes it quite useful to gene mappers trying to fit in new markers or pinpoint genes involved in diseases, although the ultimate goal--sequencing the entire human genome--will require maps with four times as many landmarks.
The completion of the two Human Genome Projects in 2001 implemented the ultimate goal of sequencing the whole human genome.[ 1, 2] This milestone not only provided a reference genome, but also unexpected opportunities, such as new questions, goals, and hopes, increased demands for improvements in the cost efficiency and throughput of DNA sequencing to an astonishing extent.
The ultimate crossover episode ever?
Because the source of heritability is inherited differences in DNA sequence, the ultimate test of the accuracy of heritability estimates is the identification of all of the DNA sequence variation responsible for heritability.
The ultimate discipline in this sequence deals with the classification of sciences and the principles of style of scientific writing that should lead to the composition of a series of scientific treatises forming an encyclopedia.
The ultimate goal of this sequencing project was to identify a wide diversity of candidate genes involved in developmental processes.
