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Population structure for the two samples was assessed using STRUCTURE V2.3.1 [17]–[18], based on the 25 microsatellite markers.
In other cases, the log of the RPKM fold-change for each gene between the two samples was assessed for the major genes (genes expressed in both samples, with an RPKM higher than a threshold value in at least one sample).
The log ratios between two samples were assessed from the respective spot signals, normalized by local weighted scatter plot smooth (LOWESS) based on print-tip.
Nineteen samples were assessed for plasma (CD138) and B-cell (CD19) markers.
Ten samples were assessed for silica content using SEM (as above).
For plasma measurements of GH and corticosterone, five to seven samples were assessed for each experimental group.
Depressive symptoms in three samples are assessed using grade-of-membership analysis to clarify the distribution of depressive symptoms across traditional affective diagnoses.
In addition, using tissue microarray, nine FTC, five FAD and five CTH samples were assessed.
Analyses were conducted with non-parametric tests: differences between two independent samples were assessed with Mann–Whitney U-test, and multiple comparisons were made with Kruskal Wallis test.
For each group at the 24 and 72 hr after treatment time points, three independent samples were assessed.
Three reference samples were assessed for accuracy studies and a relative deviation <5% was obtained.
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