To reach such extreme temperatures, the researchers are combining two previously demonstrated techniques-optical trapping and optical damping.
Two previously demonstrated and six novel, potential physical interactions were identified through the Y2H screens (Additional file 4).
This device covers the gap in terms of processing bandwidth between the two previously-demonstrated all-fiber designs, namely the fiber Bragg grating-based differentiator (<20 GHz) and that based on a uniform long-period fiber grating (>100 200 GHz).
Despite the two previously cited papers demonstrating increased radiosensitivity of HPV/p16-positive cells [ 34, 35], there is no clear evidence for the implication of viral oncoproteins in radioresponse, and data published so far are inconclusive or even sometimes conflicting.
Results shown in Fig. 3 confirmed the nectary-enriched expression for genes represented by eight contig sequences [with two others previously demonstrated: orthologs to At2g36190 [16] and At1g77110 (Ruhlmann and Carter, in preparation)], and suggest they are true orthologs to the Arabidopsis nectary-expressed genes.
The most consistent results across tissues, obtained by both methods, were those of two of the genes previously demonstrated to escape X inactivation, Eif2s3x and Utx, and for a new candidate gene (2610029G23Rik).
To investigate the catalytic promiscuity of ProcM, we chose four prochlorosins previously demonstrated to be produced by the organism for detailed structural characterization.
Because the LC-MS method does not distinguish l-2HG and d-2HG, we also analyzed the reaction mixture by GC-MS following a two step derivatization previously demonstrated to separate the two enantiomers, with the PHGDH-derived 2HG coeluting with the d-2HG but not l-2HG standard.
"We've shown that they can predict others' behaviors, which is a sophisticated ability," and one not previously demonstrated.
Furthermore, combinations of human anti-SEB monoclonal antibodies may be more effective than one, as previously demonstrated with monoclonal antibodies specific for Clostridium botulinum neurotoxin A [49] and tetanus toxin [50].
Interestingly, among the 27 up-regulated PSP genes (see Additional file 9) predicted to be targeted by down-regulated miRNAs, six were previously demonstrated to be involved in neuronal differentiation and synaptogenesis (Cdk5 [ 46], Gprin1 [ 47], Atp1a2 [ 48], Cend1 [ 49], Cacng2 [ 50] and Myo6 [ 51]).
