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Differences between the two groups were validated for four of the seven miRNAs (mir-198, −374b, −218, −149) real-time PCR (p = 0.0015 0.0289) (Additional file 3: Figure S2), yielding a specificity of 57 %.
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Molecular classification into the two groups was validated by the findings of increased hepatic population of lymphocyte subsets or tissue accumulation of matrix substrates.
The table also shows in which genes differences in expression between the two survival groups were validated using RT-PCR.
Of the pairs of genes which resulted in four sample groups that were all validated in the training dataset, no pair's groups were validated at the α = 0.05 significance level in all of the validation datasets.
Two from the "high" group were validated.
To confirm and further validate our data, IgG levels against few test antigens included in the three groups were analyzed by standard methods.
In contrast, a concerted-stepwise (SN2@P4 SN2I@P4) mechanism for 1b, bearing two bulky –tBu groups, was validated.
Eight CNVRs among the ones present in the largest number of animals (four CNVRs specific for the high and four CNVRs for the low fertility group) were validated by quantitative real-time PCR (qPCR).
Three out of the four variants were validated for CASP8.
Of the five additional genes tested, three were validated.
The four genes from this group that were validated showed strong correlation between RT-PCR and microarray results.
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