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First, spanning of the two DAD domains by the N-terminus, in essentially any plausible conformation or orientation would block addition of more than one or two actin subunits in a nascent filament or nucleus (Figure 6B).
The common and most likely mechanism derived from the above considerations invokes inhibition of filament binding by the dimeric DID-DD N-terminus when tethered to the two DAD elements emanating from the FH2 dimer.
The latter contained only the two dad regulatory elements.
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