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SSR frequencies in the two clustered and one non-clustered EST datasets of cucumber were comparable for tri-, hepta, hepta- and octanucleotides, whereas a higher frequency of dinucleotides was found in clustered ESTs (16.3 vs 12.9 for WI 1983, Table 1).
Another such gene cluster is found on chromosome 4 composed of ZEP1 and VDL2, and also in this case the transcript levels of the two clustered genes were comparable (Fig. 7A,F,G).
In contrast to these potential virulence-associated genes, the two "clustered regularly interspaced short palindromic repeats" (CRISPR -associated genes CRISPR -associatedsigenescas1ly andocas2ed with careiage significantlye sassociatedlation.
Thus, such lethal genes are likely to be responsible for the observed distorted segregation of the two clustered SSRs Hi07d08 and NZmsCN879773.
PMF search was performed as described in [ 83] on SwissProt and on the two clustered EST M. truncatula database available online (http://medicago.toulouse.inra.fr/Mt/EST/DOC/MtB.html) according to [ 84].
The two clustered genes Bv_dxny and Bv_zguf (both in the defence-related clade 7) showed different expression profiles with Bv_zguf mainly expressed in seedlings and seeds and Bv_dxny further expressed in leaves.
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