V region affinity was not affected by CH region glycosylation whereas heterologous C region of the same isotype altered the Ab binding affinity and the specificity for self-antigens.
NikR has two binding affinities for the nik operon: a nanomolar dissociation constant with stoichiometric nickel and a picomolar dissociation constant with excess nickel [Bloom, S. L., and Zamble, D. B. (2004) Biochemistry 43, 10029−10038; Chivers, P. T., and Sauer, R. T. (2002) Chem. Biol. 9, 1141−1148].
The two binding sites have different affinity towards starch, site one having low affinity12.
Because of the wide separation of the resolved affinities, the two binding events occur in sequence.
If the binding were at all cooperative or the two binding events were closer in affinity, a mixture of 1 1 and 2 1 complexes would be observed at substoichiometric protein concentrations.
Both show very close molecular structures and each one is representative of one of the two differential binding affinity groups discussed above.
Despite the use of an identical receptor, the binding mechanisms for the two ligands differ, and the binding affinities between the individual ligand and receptor proteins vary dramatically (by a factor of 200 1000).
The binding affinities of two of the six hit fragments, F090 and F152, were determined by measurement of the STD factor variation upon titration.
In MRSA, exposure to β-lactams inactivates the four high-binding-affinity PBPs normally present.
The fluorescence intensities of the binding affinities between FITC-ZE2 and E2-expressing cells were aptamer dose-dependent (Fig. 3C), and a much smaller fluorescence intensity was observed when using the FITC-ZE2-mut (Fig. 3C).
Mint1 MBD was bound at the same molar concentration of syntaxin to allow for a direct comparison between the binding affinities for the two proteins.
