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The two accessions of O. nivara formed different haplotypes, H12 and H13, and were split from Cluster B1 because of diverse nucleotide sequences in both upstream and genic regions (Figs. 4 and 5).
Again, the two accessions of Y. sedellus do not form a grade or clade.
These species are primarily Eurasian, except for one of the two accessions of E. lanceolatus.
This showed that differences between the two accessions was a greater source of gene expression variance than treatment effect (Figure 2)[40].
Using the data on polymorphism from the Nordborg lab, we selected SNPs that were known to be polymorphic between the two accessions.
110 markers (33.1 %) were polymorphic between the two accessions.
Therefore, constitutive gene expression was similar between the two accessions before As treatment.
The two accessions causing the incompatibility were removed from the data set in further analyses.
Some secondary metabolites, such as flavonoids, were also up-regulated in the two accessions.
However, over-represented GO terms unique to each of the two accessions were also found.
The data may explain the differences in As toxicity and tolerance between the two accessions.
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